Protective Action of Flavonoids Genistein and Gingerol against Cisplatin Toxicity In Vitro

Human lymphocyte culture of two healthy donors was carried out.[3] Later, metaphases were examined for different types of aberrations. For sister chromatid exchange analysis, bromodeoxyuridine (10 μg/ml) was added at the beginning of the culture.[4] Cell cycle kinetics was studied by the BrdU‐Harlequin technique using the replication index (RI) calculated by the standard formula.[5] The Student ‘t’-test was used for statistical analysis using ‘Statistica’ software (StatSoft, Inc.).